QUANTIZZAZIONE DI HBV-DNA IN REAL-TIME PCR: COBAS AMPLIPREP™/COBAS TAQMAN™ HBV TEST.
نویسندگان
چکیده
منابع مشابه
COBAS AmpliPrep-COBAS TaqMan hepatitis B virus (HBV) test: a novel automated real-time PCR assay for quantification of HBV DNA in plasma.
Success in antiviral therapy for chronic hepatitis B is supported by highly sensitive PCR-based assays for hepatitis B virus (HBV) DNA. Nucleic acid extraction from biologic specimens is technically demanding, and reliable PCR results depend on it. The performances of the fully automatic system COBAS AmpliPrep-COBAS TaqMan 48 (CAP-CTM; Roche, Branchburg, NJ) for HBV DNA extraction and real-time...
متن کاملComparison of the AdvanSure HBV real-time PCR test with three other HBV DNA quantification assays.
BACKGROUND We compared the AdvanSure hepatitis B virus real-time polymerase chain reaction (AdvanSure HBV) kit with three other HBV DNA quantification assays and evaluated its performance. METHODS The AdvanSure HBV real-time PCR assay was compared with the Abbott RealTime HBV Quantification Kit, the COBAS TaqMan HBV Test, and the VERSANT HBV branched DNA 3.0 assay. The precision, linearity, a...
متن کاملComparison of the Cobas Ampliprep/Cobas TaqMan HBV Test versus the Cobas Amplicor HBV monitor for HBV-DNA detection and quantification during antiviral therapy.
Performances of the new automatic system COBAS AmpliPrep/COBAS TaqMan 48 (CAP/CTM) (Roche, Branchburg, NJ) for HBV DNA extraction and real-time PCR quantification were assessed and compared with the endpoint PCR COBAS AMPLICOR HBV Monitor (CAHBM, Roche). Analytical evaluation with proficiency panels from UK National External Quality Assessment Scheme (UK NEQAS) over a 1-year period of distribut...
متن کاملDynamic comparison between Daan real-time PCR and Cobas TaqMan for quantification of HBV DNA levels in patients with CHB
BACKGROUND Hepatitis B virus (HBV) DNA levels are crucial for managing chronic hepatitis B (CHB). It was unclear whether Daan real-time polymerase chain reaction test (Daan test) or COBAS TaqMan HBV DNA Test (Cobas TaqMan) was superior in measuring different HBV DNA levels in clinical specimens. METHODS We enrolled 67 treatment-naïve, HBV surface antigen-positive CHB patients (high baseline v...
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چکید ه سابقه و هدف بتادومیکروگلوبولین ( b 2MG) ، زنجیره سبک آنتی‌ژن MHC-I می‌باشد که غلظت سرمی آن ( S β 2MG ) در افراد سالم کمتر از mg/L 3 ذکر شده است. در عفونت‌های هپاتیتی، عرضه آنتی‌ژن‌های ویروسی سطح هپاتوسیت‌ها روی آنتی‌ژن‌های MHC-I در حذف ویروس نقش مهمی ایفا می‌کند. مواد و روش‌ها در این مطالعه توصیفی درسرم 4 9 بیمار مبتلا به هپاتیت B در مقایسه با 3 5 فرد کنترل با سرولوژی منفی (فا...
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ژورنال
عنوان ژورنال: Microbiologia Medica
سال: 2006
ISSN: 2280-6423
DOI: 10.4081/mm.2006.3361